Advanced Separation Science

Overview

Summary of Lecture Content:

• Introduction to separations and chromatography
• Liquid Chromatography (1)
• Liquid Chromatography (2)
• Liquid Chromatography (3)
• Multi-dimensional Chromatography
• Gas Chromatography
• TLC/IC
• Biopharmaceutical Analysis
• Sample preparation (1)
• Sample preparation (2)
• Capillary Electrophoresis (1)
• Capillary Electrophoresis (2)

Summary of Practical Content:

• Practical 1: HPLC method development.
• Practical 2: HPLC method validation.
• Practical 3: Gas chromatography.

Learning Objectives

At the end of the module students will be be able to:
• Critically evaluate the principles and applications of modern separation techniques, including
liquid chromatography, gas chromatography, capillary electrophoresis, and multidimensional
approaches, within the context of pharmaceutical analysis.
• Design and optimise chromatographic methods for qualitative and quantitative pharmaceutical
analysis, considering factors such as resolution, selectivity, sensitivity, and efficiency.
• Apply advanced sample preparation techniques to improve analytical performance, particularly
in the context of complex matrices such as biological samples and biopharmaceutical products.
• Interpret and critically assess chromatographic and electrophoretic data, including the use of
method validation parameters (e.g., accuracy, precision, specificity, linearity, LOD/LOQ).
• Demonstrate practical proficiency in operating key analytical instruments (e.g. HPLC, GC),
performing method development and validation in a laboratory setting.
• Communicate analytical findings effectively, using appropriate scientific language and data
presentation techniques, in both written and verbal formats.

Skills

Skills associated with module:

• Ability to select, adapt, and troubleshoot analytical techniques to solve complex problems in
pharmaceutical analysis.
• Proficient use of chromatographic and electrophoretic instrumentation, including method
development, validation, and data analysis.
• Capacity to critically assess experimental data and literature and effectively communicate
findings through reports and presentations.

Assessment

Assessment Profile
Element type Element weight (%)
1. Coursework 100

Course Requirements:
Coursework submission 100 %
Laboratory Class attendance 100 %
Total coursework elements must be passed at 50%.